Regulation of Pygopus (hPygo2) expression in prostate cancer by E74-Like factor 1 (Elf-1) transcription factor

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dc.contributor.authorGonzalez Aguirre, Paola
dc.date.issued2016-05
dc.description.abstractProstate cancer is a worldwide health concern. Pygopus2 (hPygo2) protein is required for growth in breast, ovarian, cervical and prostate cancer. hPygo2 expression is regulated by the Rb protein via the ETS factor Elf-1 in cervical and breast cancer. Additionally, the ETS family has confirmed roles in carcinogenesis and proliferation. The mechanism of hPygo2 expression has not been elucidated in prostate cancer. My hypothesis proposes that hPygo2 expression is regulated by Elf-1 bound to its promoter region. Prostate cancer cell lines were used to show protein levels of hPygo2, Elf-1 and ETS. ChIP assays confirmed varying binding capability of Elf-1 and ETS factors to the proximal promoter region between cell lines. Elf-1 knockdown experiments were performed, results show no change in hPygo2 protein levels but show reduction in 22Rv1 mRNA levels. These results suggest that Elf-1 might not be exclusively involved in the activation of Pygopus expression in prostate cancer.
dc.description.noteIncludes bibliographical references (pages 57-71).
dc.format.extentxii, 80 pages : illustrations (chiefly color)
dc.format.mediumText
dc.identifier.urihttps://hdl.handle.net/20.500.14783/13709
dc.language.isoen
dc.publisherMemorial University of Newfoundland
dc.rights.licenseThe author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
dc.subjectPygopus
dc.subjectProstate Cancer
dc.subjectCancer
dc.subjectETS factors
dc.subjectElf-1
dc.subjecthPygo2
dc.subjectProstate
dc.subject.lcshProstate--Cancer--Genetic aspects
dc.subject.lcshCellular signal transduction
dc.subject.lcshTranscription factors
dc.subject.lcshCancer cells--Growth--Regulation
dc.subject.meshProstate Neoplasms--genetics
dc.subject.meshSignal Transduction
dc.subject.meshTranscription Factors
dc.subject.meshIntracellular Signaling Peptides and Proteins
dc.titleRegulation of Pygopus (hPygo2) expression in prostate cancer by E74-Like factor 1 (Elf-1) transcription factor
dc.typeMaster thesis
mem.campusSt. John's Campus
mem.convocationDate2016-05
mem.departmentBioMedical Sciences
mem.divisionsBiomedical
mem.facultyFaculty of Medicine
mem.fullTextStatuspublic
mem.institutionMemorial University of Newfoundland
mem.isPublishedunpub
mem.thesisAuthorizedNameGonzalez Aguirre, Paola
thesis.degree.disciplineBioMedical Sciences
thesis.degree.grantorMemorial University of Newfoundland
thesis.degree.levelmasters
thesis.degree.nameM. Sc. Med.

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